Medium data

Search for medium no. = [1000].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1000   PYVG MEDIUM

Trypticase peptone (BD-BBL)  5.0     g
Bacto peptone (BD-Difco)  5.0     g
Yeast extract (BD-Difco)  10.0     g
Glucose  10.0     g
0.05% Resazurin solution  1.0     ml
Hemin solution (see Medium No. 469)  10.0     ml
Menadione solution (see Medium No. 469)  10.0     ml
VFA solution (see Medium No. 133)  3.1     ml
Salt solution (see below)  40.0     ml
L-Cysteine·HCl·H2O  0.5     g
Distilled water  1.0     L
Adjust pH to 7.0.
Prepare medium anaerobically under a 100% N2 gas-atmosphere. Dispense in anaerobic vials (1/3 vol.) and autoclave.
Salt solution:
CaCl2·2H2O  0.20    g
MgSO4·7H2O  0.48    g
K2HPO4  1.0     g
KH2PO4  1.0     g
NaHCO3  10.0     g
NaCl  2.0     g
Distilled water  1.0     L



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