1000 PYVG MEDIUM
| Trypticase peptone (BD-BBL) | 5.0 | g |
| Bacto peptone (BD-Difco) | 5.0 | g |
| Yeast extract (BD-Difco) | 10.0 | g |
| Glucose | 10.0 | g |
| 0.05% Resazurin solution | 1.0 | ml |
| Hemin solution (see Medium No. 469) | 10.0 | ml |
| Menadione solution (see Medium No. 469) | 10.0 | ml |
| VFA solution (see Medium No. 133) | 3.1 | ml |
| Salt solution (see below) | 40.0 | ml |
| L-Cysteine·HCl·H2O | 0.5 | g |
| Distilled water | 1.0 | L |
| CaCl2·2H2O | 0.20 | g |
| MgSO4·7H2O | 0.48 | g |
| K2HPO4 | 1.0 | g |
| KH2PO4 | 1.0 | g |
| NaHCO3 | 10.0 | g |
| NaCl | 2.0 | g |
| Distilled water | 1.0 | L |