133 MEDIUM 10
Trypticase peptone (BD-BBL) | 2.0 | g |
Yeast extract (BD-Difco) | 0.5 | g |
0.2% Hemin solution | 1.0 | ml |
VFA solution (see below) | 3.1 | ml |
Glucose | 0.5 | g |
Cellobiose | 0.5 | g |
Soluble starch | 0.5 | g |
Salt solution No. 1 (see below) | 37.5 | ml |
Salt solution No. 2 (see below) | 37.5 | ml |
Agar | 18.0 | g |
0.1% Resazurin solution | 0.5 | ml |
10% Toray silicone SH 5535 solution | 5.0 | ml |
5% L-Cysteine·HCl·H2O solution | 10.0 | ml |
25% L-Ascorbic acid solution | 2.0 | ml |
4% Na2CO3-CO2 buffer | 100.0 | ml |
Distilled water | 850.0 | ml |
Acetic acid | 17.0 | ml |
Propionic acid | 6.0 | ml |
n-Butyric acid | 4.0 | ml |
iso-Butyric acid | 1.0 | ml |
n-Valeric acid | 1.0 | ml |
iso-Valeric acid | 1.0 | ml |
DL-α-Methyl butyric acid | 1.0 | ml |
K2HPO4 | 0.78 | g |
Distilled water | 100.0 | ml |
KH2PO4 | 0.47 | g |
NaCl | 1.18 | g |
(NH4)2SO4 | 1.2 | g |
CaCl2 | 0.12 | g |
MgSO4·H2O | 0.25 | g |
Distilled water | 100.0 | ml |