Medium data

Search for medium no. = [133].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

133   MEDIUM 10

Trypticase peptone (BD-BBL)  2.0     g
Yeast extract (BD-Difco)  0.5     g
0.2% Hemin solution  1.0     ml
VFA solution (see below)  3.1     ml
Glucose  0.5     g
Cellobiose  0.5     g
Soluble starch  0.5     g
Salt solution No. 1 (see below)  37.5     ml
Salt solution No. 2 (see below)  37.5     ml
Agar  18.0     g
0.1% Resazurin solution  0.5     ml
10% Toray silicone SH 5535 solution  5.0     ml
5% L-Cysteine·HCl·H2O solution  10.0     ml
25% L-Ascorbic acid solution  2.0     ml
4% Na2CO3-CO2 buffer  100.0     ml
Distilled water  850.0     ml
Adjust pH to 6.7-6.8.
VFA solution:
Acetic acid  17.0     ml
Propionic acid  6.0     ml
n-Butyric acid  4.0     ml
iso-Butyric acid  1.0     ml
n-Valeric acid  1.0     ml
iso-Valeric acid  1.0     ml
DL-α-Methyl butyric acid  1.0     ml
Salt solution No. 1:
K2HPO4  0.78    g
Distilled water  100.0     ml
Salt solution No. 2:
KH2PO4  0.47    g
NaCl  1.18    g
(NH4)2SO4  1.2     g
CaCl2  0.12    g
MgSO4·H2O  0.25    g
Distilled water  100.0     ml



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