JCM Catalogue

Bacillus stearothermophilus Donk 1920
Taxonomy in NCBI database: Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae.
2501 <-- ATCC 12980 <-- Natl. Canner's Assoc., Washington; NCA 26.
Accessioned in 1984.
=ATCC 12980 =BCRC 11092 =CCM 2062 =CCUG 26241 =CIP 66.23 =DSM 22 =IAM 11062 =IFO 12550 =KCTC 1752 =KCTC 1665 =LMG 6939 =NBRC 12550 =NCFB 1768 =NCIMB 8923 =NCTC 10339 =NRRL B-1172 =VKM B-510 =VTT E-81128.
Bacillus stearothermophilus.
Type strain [596].
Medium: 22;  Temperature: 55°C; Rehydration fluid: 663.   more information +  Full / Short version of the cataloguepage top
Biochemistry/Physiology: [1693,3056].
Numerical taxonomy: API [2275], [3056].
Cell wall: [251].
Enzyme electrophoretic profile: [1702].
G+C (mol%): 51.5 (Bd), 51.9 (Tm) [2214], 51.6 (Tm) [3056].
Phylogeny: 16S rRNA gene (X60640, LC177207) [3047].
Taxonomy: [4990].
Genome sequence: JYNW00000000.
Application: Biological indicator for moist heat sterilization (Terminal sterilization and sterilization indicators, Japanese Pharmacopoeia, 16 ed.).
NCBI Assembly ID: GCA_001277805 (GenBank), GCF_001277805 (RefSeq).
BacDive ID: 1419.
NCBI Taxonomy ID: 1422.
Publication(s) using this strain [A06198, A08254, B11200, A14168, A15170].
Patent publication(s) using this strain [JP2007-143493A, JP2008-048652A, JP2008-048703A, JP2008-289398A, JP2008-289419A, JP2014-122213A, 2019-156695].
 Related information on delivery / use of the strain
Biosafety level 1
Terms and conditions Not applicable
Export control (1) No
Distribution control in Japan (2) No
Genetically modified microorganism No
Technical information -
Additional information -
 (1) in complying with the Foreign Exchange and Foreign Trade Control Law of Japan
 (2) in complying with the Plant Protection Law of Japan

 Delivery category
Domestic A (Freeze-dried or L-dried culture) or C (Actively growing culture on request)
Overseas A (Freeze-dried or L-dried culture) or C (Actively growing culture on request)
Viability and purity assays of this product were performed at the time of production as part of quality control. The authenticity of the culture was confirmed by analyzing an appropriate gene sequence, e.g., the 16S rRNA gene for prokaryotes, the D1/D2 region of LSU rRNA gene, the ITS region of the nuclear rRNA operon, etc. for eukaryotes. The characteristics and/or functions of the strain appearing in the catalogue are based on information from the corresponding literature and JCM does not guarantee them.
9488 <-- ATCC 7953 <-- C. P. Hegarty NCA 1518.
Accessioned in 1994.
=ATCC 7953 =BCRC 10285 =CCUG 621 =CIP 52.81 =DSM 5934 =IAM 11002 =IFO 13737 =LMG 13578 =NBRC 13737 =NCFB 733 =NCIMB 8157 =NCIMB 8925 =NCTC 10007 =VKM B-715.
Bacillus stearothermophilus.
Medium: 22;  Temperature: 55°C; Rehydration fluid: 663.   more information +
Source: Under-processed canned food.
Genome sequence: JALS00000000.
Application: Biological indicator for moist heat sterilization (Terminal sterilization and sterilization indicators, Japanese Pharmacopoeia, 16 ed.).
NCBI Assembly ID: GCA_000705495 (GenBank), GCF_000705495 (RefSeq).
BacDive ID: 1420.
Publication(s) using this strain [A06029, A07271].
Patent publication(s) using this strain [2017-169505].
 Related information on delivery / use of the strain
Biosafety level 1
Terms and conditions Not applicable
Export control (1) No
Distribution control in Japan (2) No
Genetically modified microorganism No
Technical information -
Additional information -
 (1) in complying with the Foreign Exchange and Foreign Trade Control Law of Japan
 (2) in complying with the Plant Protection Law of Japan

 Delivery category
Domestic A (Freeze-dried or L-dried culture) or C (Actively growing culture on request)
Overseas A (Freeze-dried or L-dried culture) or C (Actively growing culture on request)
Viability and purity assays of this product were performed at the time of production as part of quality control. The authenticity of the culture was confirmed by analyzing an appropriate gene sequence, e.g., the 16S rRNA gene for prokaryotes, the D1/D2 region of LSU rRNA gene, the ITS region of the nuclear rRNA operon, etc. for eukaryotes. The characteristics and/or functions of the strain appearing in the catalogue are based on information from the corresponding literature and JCM does not guarantee them.
11297 <-- ATCC 10149 <-- N. R. Smith T15 <-- Curran <-- Iowa State Coll., USA.
Accessioned in 2001.
=ATCC 10149 =CGMCC 1.6483 =CIP 105453 =DSM 6790 =IAM 11004.
Bacillus stearothermophilus.
"Bacillus calidolactis".
Medium: 22;  Temperature: 55°C; Rehydration fluid: 663.   more information +
BacDive ID: 1421.
 Related information on delivery / use of the strain
Biosafety level 1
Terms and conditions Not applicable
Export control (1) No
Distribution control in Japan (2) No
Genetically modified microorganism No
Technical information -
Additional information -
 (1) in complying with the Foreign Exchange and Foreign Trade Control Law of Japan
 (2) in complying with the Plant Protection Law of Japan

 Delivery category
Domestic A (Freeze-dried or L-dried culture) or C (Actively growing culture on request)
Overseas A (Freeze-dried or L-dried culture) or C (Actively growing culture on request)
Viability and purity assays of this product were performed at the time of production as part of quality control. The authenticity of the culture was confirmed by analyzing an appropriate gene sequence, e.g., the 16S rRNA gene for prokaryotes, the D1/D2 region of LSU rRNA gene, the ITS region of the nuclear rRNA operon, etc. for eukaryotes. The characteristics and/or functions of the strain appearing in the catalogue are based on information from the corresponding literature and JCM does not guarantee them.
12216 <-- CIP 67.5 <-- J. O. Clair C 953.
Accessioned in 2003.
=CIP 67.5 =DSM 1550 =IAM 12043 =JCM 14450 =NBIMCC 2330 =NBRC 100862 =NCDO 1780 =NCIMB 11780.
Bacillus stearothermophilus.
"Bacillus stearothermophilus var. calidolactis".
Medium: 75;  Temperature: 55°C; Rehydration fluid: 663.   more information +
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Source: Evaporated milk.
Phylogeny: 16S rRNA gene (AB598739).
Assay: Benzylpenicillin.
BacDive ID: 1448.
 Related information on delivery / use of the strain
Biosafety level 1
Terms and conditions Not applicable
Export control (1) No
Distribution control in Japan (2) No
Genetically modified microorganism No
Technical information -
Additional information -
 (1) in complying with the Foreign Exchange and Foreign Trade Control Law of Japan
 (2) in complying with the Plant Protection Law of Japan

 Delivery category
Domestic A (Freeze-dried or L-dried culture) or C (Actively growing culture on request)
Overseas A (Freeze-dried or L-dried culture) or C (Actively growing culture on request)
Viability and purity assays of this product were performed at the time of production as part of quality control. The authenticity of the culture was confirmed by analyzing an appropriate gene sequence, e.g., the 16S rRNA gene for prokaryotes, the D1/D2 region of LSU rRNA gene, the ITS region of the nuclear rRNA operon, etc. for eukaryotes. The characteristics and/or functions of the strain appearing in the catalogue are based on information from the corresponding literature and JCM does not guarantee them.
12894 <-- H. Takami HTA462.
Accessioned in 2004.
Bacillus stearothermophilus.
Medium: 443;  Temperature: 60°C; Rehydration fluid: 7.   more information +
Source: Mud from the Challenger Deep in the Mariana Trench (10,897 m in depth) [5857].
Biochemistry/Physiology: [5858].
Fatty acid: [5858].
Quinone: MK-7 [5858].
G+C (mol%): 51.8 (HPLC) [5858].
DNA-DNA relatedness: [5858].
Phylogeny: 16S rRNA gene (AB002646) [5857].
More information: λ-Phage [5858].
BacDive ID: 161282.
Publication(s) using this strain [A08174].
 Related information on delivery / use of the strain
Biosafety level 1 -
Terms and conditions Imposed
Export control (1) No -
Distribution control in Japan (2) No -
Genetically modified microorganism No -
Technical information - -
Additional information - -
 (1) in complying with the Foreign Exchange and Foreign Trade Control Law of Japan
 (2) in complying with the Plant Protection Law of Japan

 Delivery category
Domestic A (Freeze-dried or L-dried culture) or C (Actively growing culture on request)
Overseas A (Freeze-dried or L-dried culture) or C (Actively growing culture on request)
Viability and purity assays of this product were performed at the time of production as part of quality control. The authenticity of the culture was confirmed by analyzing an appropriate gene sequence, e.g., the 16S rRNA gene for prokaryotes, the D1/D2 region of LSU rRNA gene, the ITS region of the nuclear rRNA operon, etc. for eukaryotes. The characteristics and/or functions of the strain appearing in the catalogue are based on information from the corresponding literature and JCM does not guarantee them.
14450 <-- IAM 12043 <-- M. Iwaida <-- Neth. Inst. voor Zwnelonderzoek; C 953.
Accessioned in 2006.
=CIP 67.5 =DSM 1550 =IAM 12043 =JCM 12216 =NBIMCC 2330 =NBRC 100862 =NCDO 1780 =NCIMB 11780.
Bacillus stearothermophilus.
"Bacillus stearothermophilus var. calidolactis".
Medium: 75;  Temperature: 55°C; Rehydration fluid: 663.   more information +
Source: Evaporated milk.
Phylogeny: 16S rRNA gene (AB598739).
Assay: Penicillins in milk [6518].
Genomic DNA is available from RIKEN BRC-DNA Bank: JGD07087.
 Related information on delivery / use of the strain
Biosafety level 1
Terms and conditions Not applicable
Export control (1) No
Distribution control in Japan (2) No
Genetically modified microorganism No
Technical information -
Additional information -
 (1) in complying with the Foreign Exchange and Foreign Trade Control Law of Japan
 (2) in complying with the Plant Protection Law of Japan

 Delivery category
Domestic A (Freeze-dried or L-dried culture) or C (Actively growing culture on request)
Overseas A (Freeze-dried or L-dried culture) or C (Actively growing culture on request)
Viability and purity assays of this product were performed at the time of production as part of quality control. The authenticity of the culture was confirmed by analyzing an appropriate gene sequence, e.g., the 16S rRNA gene for prokaryotes, the D1/D2 region of LSU rRNA gene, the ITS region of the nuclear rRNA operon, etc. for eukaryotes. The characteristics and/or functions of the strain appearing in the catalogue are based on information from the corresponding literature and JCM does not guarantee them.
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