Medium data

Search for medium no. = [993].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

993   ANAEROBIC ALKALINE YE MEDIUM

NaCl  2.0     g
NH4Cl  1.0     g
KH2PO4  0.1     g
K2HPO4  0.1     g
KCl  0.1     g
MgCl2·6H2O  0.1     g
Tris base  3.0     g
Na2S2O3·5H2O  3.0     g
Trace element solution (see Medium No. 439)  1.0     ml
Resazurin  1.0     mg
Distilled water  900.0     ml
Mix components thoroughly, adjust pH to 9.0 with NaOH and autoclave under a N2 atmosphere. After cooling, replace the gas phase with a N2-CO2 (4:1, v/v) gas mixture. Add the following solutions from sterile anaerobic stocks (autoclaved and stored under a N2 atmosphere):
10% (w/v) Yeast extract solution  20.0     ml
8% NaHCO3 solution  30.0     ml
5% Na2S·9H2O solution  8.0     ml