Medium data
Search for medium no. = [989].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
989 IRD AMINOBACTERIUM MEDIUM
| NH4Cl | 1.0 | g |
| KH2PO4 | 0.3 | g |
| K2HPO4 | 0.3 | g |
| NaCl | 20.0 | g |
| KCl | 0.1 | g |
| CaCl2·2H2O | 0.1 | g |
| L-Serine | 1.0 | g |
| Yeast extract (BD-Difco) | 2.0 | g |
| Trace element solution (see Medium No. 439) | 1.0 | ml |
| Selenite tungstate solution (see Medium No. 431) | 1.0 | ml |
| L-Cysteine·HCl·H2O | 0.5 | g |
| Resazurin | 0.5 | mg |
| Distilled water | 945.0 | ml |
Mix components thoroughly and adjust pH to 7.2. Bring to a boil, cool down under a N2-CO2 (4:1, v/v) gas stream, seal with butyl rubber stoppers and autoclave. Aseptically and anaerobically add the following stock solutions [autoclaved and stored under a N2 atmosphere or *filter-sterilize and store under a N2-CO2 (4:1, v/v) gas mixture]:
| 15% MgCl2·6H2O solution | 20.0 | ml |
| 8% NaHCO3 solution* | 25.0 | ml |
| 5% Na2S·9H2O solution | 10.0 | ml |
Readjust pH to 7.2, if necessary.
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