Medium data

Search for medium no. = [989].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

989   IRD AMINOBACTERIUM MEDIUM

NH4Cl  1.0     g
KH2PO4  0.3     g
K2HPO4  0.3     g
NaCl  20.0     g
KCl  0.1     g
CaCl2·2H2O  0.1     g
L-Serine  1.0     g
Yeast extract (BD-Difco)  2.0     g
Trace element solution (see Medium No. 439)  1.0     ml
Selenite tungstate solution (see Medium No. 431)  1.0     ml
L-Cysteine·HCl·H2O  0.5     g
Resazurin  0.5     mg
Distilled water  945.0     ml
Mix components thoroughly and adjust pH to 7.2. Bring to a boil, cool down under a N2-CO2 (4:1, v/v) gas stream, seal with butyl rubber stoppers and autoclave. Aseptically and anaerobically add the following stock solutions [autoclaved and stored under a N2 atmosphere or *filter-sterilize and store under a N2-CO2 (4:1, v/v) gas mixture]:
15% MgCl2·6H2O solution  20.0     ml
8% NaHCO3 solution*  25.0     ml
5% Na2S·9H2O solution  10.0     ml
Readjust pH to 7.2, if necessary.