Medium data

Search for medium no. = [987].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.


NH4Cl  1.0     g
K2HPO4  0.5     g
CaCl2·2H2O  0.1     g
KCl  0.8     g
Sodium acetate  0.5     g
NaCl  200.0     g
Yeast extract (BD-Difco)  1.0     g
Tryptone (BD-Difco)  0.5     g
Trace element solution (see Medium No. 439)  1.0     ml
L-Cysteine·HCl·H2O  0.5     g
Resazurin  0.5     mg
Distilled water  855.0     ml
Mix components thoroughly, adjust pH to 7.6 with KOH and autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, add the following solutions from anaerobic stocks (autoclaved or *filter-sterilized):
8% NaHCO3 solution*  25.0     ml
1 M Glucose solution  20.0     ml
10% MgSO4·7H2O  50.0     ml
10% Tryptone solution  50.0     ml
Aseptically distribute the medium into culture vessels under a stream of N2-CO2 (4:1, v/v), and seal with butyl rubber stoppers. Prior to inoculation, add per liter the following solution from an anaerobic stock (autoclave and store under a N2 atmosphere).
5% Na2S·9H2O solution  8.0     ml

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