Medium data

Search for medium no. = [979].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

979   M13 MEDIUM

Peptone  0.25    g
Yeast extract  0.25    g
Glucose  0.25    g
Vitamin solution (see below)  10.0     ml
Hutner's basal salts (see below)  20.0     ml
0.1 M Tris-HCl buffer (pH 7.5)  50.0     ml
Artificial seawater  500.0     ml
Distilled water  420.0     ml
Vitamin solution:
Vitamin B12  0.1     mg
Biotin  2.0     mg
Thiamine·HCl·2H2O  5.0     mg
Calcium pantothenate  5.0     mg
Folic acid  2.0     mg
Riboflavin  5.0     mg
Nicotinamide  5.0     mg
Distilled water  1.0     L
Filter-sterilize.
Hutner's basal salts:
Nitrilotriacetic acid (NTA)  10.0     g
MgSO4·7H2O  29.7     g
CaCl2·2H2O  3.335   g
(NH4)6MoO24·4H2O  9.25    mg
FeSO4·7H2O  99.0     mg
Metals ''44'' (see Medium No. 149)  50.0     ml
Distilled water  950.0     ml
Dissolve nitrilotriacetic acid and adjust pH to 7.0 with KOH (about 7.3 g). Then proceed to add salts. Readjust pH to 6.8 with NaOH or H2SO4.