Medium data
Search for medium no. = [978].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
978 THERMOCRINIS JAMIESONII MEDIUM
| NaCl | 3.0 | g |
| KCl | 0.15 | g |
| Na2SO4 | 0.3 | g |
| MgSO4·7H2O | 0.123 | g |
| CaCl2·2H2O | 0.015 | g |
| NH4Cl | 0.107 | g |
| NaH2PO4 | 1.38 | g |
| Mineral solution (see Medium No. 976 | 5.0 | ml |
| Distilled water | 1.0 | L |
Mix components thoroughly and adjust pH to 7.25 with NaOH. Distribute the medium into culture vessels (e.g., 5.0 ml in 25 ml serum bottles/Balch tubes) under a stream of N2, seal with butyl rubber stoppers and autoclave. After cooling, aseptically add per liter the following solutions (autoclaved or *filter-sterilized and stored anaerobically):
| 0.2 M Sodium acetate solution | 10.0 | ml |
| 0.2 M Sodium thiosulfate solution | 10.0 | ml |
| 8.0% (w/v) NaHCO3 solution* | 10.0 | ml |
Before inoculation, add filter-sterilized O2 gas or air, at a final concentration of 2% O2 in the gas phase.
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