Medium data

Search for medium no. = [978].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

978   THERMOCRINIS JAMIESONII MEDIUM

NaCl  3.0     g
KCl  0.15    g
Na2SO4  0.3     g
MgSO4·7H2O  0.123   g
CaCl2·2H2O  0.015   g
NH4Cl  0.107   g
NaH2PO4  1.38    g
Mineral solution (see Medium No. 976  5.0     ml
Distilled water  1.0     L
Mix components thoroughly and adjust pH to 7.25 with NaOH. Distribute the medium into culture vessels (e.g., 5.0 ml in 25 ml serum bottles/Balch tubes) under a stream of N2, seal with butyl rubber stoppers and autoclave. After cooling, aseptically add per liter the following solutions (autoclaved or *filter-sterilized and stored anaerobically):
0.2 M Sodium acetate solution  10.0     ml
0.2 M Sodium thiosulfate solution  10.0     ml
8.0% (w/v) NaHCO3 solution*  10.0     ml
Before inoculation, add filter-sterilized O2 gas or air, at a final concentration of 2% O2 in the gas phase.



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