Medium data
Search for medium no. = [976].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
976 THERMOFLEXUS HUNGENHOLTZII MEDIUM
| NaCl | 3.0 | g |
| KCl | 0.15 | g |
| Na2SO4 | 0.3 | g |
| MgSO4·7H2O | 0.123 | g |
| CaCl2·2H2O | 0.015 | g |
| NH4Cl | 0.107 | g |
| NaH2PO4 | 1.38 | g |
| Mineral solution (see below) | 5.0 | ml |
| Distilled water | 1.0 | L |
Mix components thoroughly and adjust pH to 6.75 with NaOH. Distribute the medium into culture vessels (e.g., 5.0 ml in 25 ml serum bottles/Balch tubes) under a stream of N2, seal with butyl rubber stoppers and autoclave. After cooling, aseptically add per liter the following solutions (filter-sterilized and stored anaerobically):
| Trace vitamins (see Medium No. 197) | 10.0 | ml |
| 10% (w/v) Peptone solution | 10.0 | ml |
Before inoculation, add filter-sterilized O2 gas or air, at a final concentration of 1% O2 in the gas phase.
Mineral solution :
| EDTA | 3.7 | g |
| FeSO4·7H2O | 1.1 | g |
| MnCl2·4H2O | 98.4 | mg |
| ZnSO4·7H2O | 28.8 | mg |
| CoCl2·6H2O | 23.8 | mg |
| CuCl2·2H2O | 17.2 | mg |
| Na2MoO4·2H2O | 24.2 | mg |
| H3BO3 | 31.0 | mg |
| Distilled water | 1.0 | L |
Adjust pH to 6.0 with KOH.
Comment: Consistent growth in serum tubes requires pretreatment of butyl rubber stoppers by boiling in 1% sodium sulfide solution (1 hr) followed by boiling in distilled H2O (1 hr, twice).
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