Medium data

Search for medium no. = [977].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

977   VNG MEDIUM

10 x CMD stock solution (see below)  100.0     ml
Trace vitamins (see Medium No. 197)  10.0     ml
NH4Cl  0.027   g
HEPES  3.57    g
Distilled water  790.0     ml
Mix components thoroughly, adjust pH to 8.1 with NaOH or HCl. Distribute the medium into culture vessels (e.g., 9 ml in 25 ml serum bottles/Balch tubes), seal with butyl rubber stoppers and autoclave. After cooling, add the following solution (per 900 ml, autoclaved):
20% (w/v) Glucose  100.0     ml
10 x CMD stock solution:
Nitrilotriacetic acid  1.0     g
5 x CMD micronutrient solution (see below)  1.0     ml
FeCl3·6H2O  4.8     mg
CaSO4·2H2O  0.6     g
MgSO4·7H2O  1.0     g
NaCl  0.8     g
KNO3  1.03    g
NaNO3  6.89    g
NaH2PO4  1.11    g
Distilled water  1.0     L
Do not use if exceeds 90 days shelf life.
5 x CMD micronutrient solution:
Distilled water  100.0     ml
6.25 N H2SO4 solution  0.25    ml
MnSO4·xH2O  1.14    g
ZnSO4·7H2O  0.25    g
H3BO3  0.25    g
CuCl2·2H2O  8.5     mg
Na2MoO4·2H2O  12.5     mg
CoCl2·6H2O  22.5     mg