Medium data

Search for medium no. = [968].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

968   MODIFIED CALDICELLULOSIRUPTOR MEDIUM

NH4Cl  0.9     g
NaCl  0.9     g
MgCl2·6H2O  0.4     g
KH2PO4  0.75    g
K2HPO4  1.5     g
Trypticase peptone (BD-BBL)  2.0     g
Yeast extract (BD-Difco)  1.0     g
Trace element solution SL-10 (see Medium No. 433)  1.0     ml
FeCl3·6H2O  2.5     mg
Xylose  1.0     g
L-Cysteine·HCl·H2O  0.75    g
Resazurin  0.5     mg
Distilled water  1.0     L
Mix components, except xylose, thoroughly and adjust pH to 7.2. Distribute the medium into culture vessels under 100% N2, seal with butyl rubber stoppers and autoclave. Add xylose after autoclaving from an anoxic stock solution prepared under a N2 gas atmosphere and sterilized by filtration.



Copyright © 2024 Microbe Division (JCM) - All Rights Reserved