Medium data

Search for medium no. = [963].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

963 DESULFOTOMACULUM TONGENSE MEDIUM

Sea salts (Sigma)	`30.0`	g
KH₂PO₄	`0.14`	g
NH₄Cl	`0.25`	g
Sodium pyruvate	`1.1`	g
Resazurin	`1.0`	mg
Distilled water	`1.0`	L

Mix components thoroughly, bring to a boil and cool down to room temperature under a N₂-CO₂ (4:1, v/v) gas stream. Dispense the medium into culture vessels under a N₂-CO₂ (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add per liter the following solutions from anaerobic stocks (autoclaved or *filter-sterilized):

Trace vitamins* (see Medium No. 197)	`10.0`	ml
8% NaHCO₃ solution*	`20.0`	ml
5% Na₂S·9H₂O solution	`10.0`	ml

Check final pH to be 7.0 - 7.2.