Medium data

Search for medium no. = [962].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

962   GAL1 MEDIUM

KH2PO4  0.3     g
NH4Cl  0.3     g
NaCl  1.0     g
KCl  0.5     g
MgCl2·6H2O  0.4     g
CaCl2·2H2O  0.15    g
Trace element solution (see Medium No. 439)  1.0     ml
Yeast extract  0.5     g
L-Cysteine·HCl·H2O  0.5     g
Resazurin  1.0     mg
Distilled water  1.0     L
Mix components thoroughly, adjust pH to 7.0 with KOH and autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, add the following solution from an anaerobic stock [filter-sterilized and stored under N2-CO2 (4:1, v/v)]:
8% NaHCO3 solution  25.0     ml
Aseptically distribute the medium into culture vessels under a stream of N2-CO2 (4:1, v/v) and seal with butyl rubber stoppers. Prior to inoculation, add per liter the following solution from an anaerobic stock (autoclave and store under a N2 atmosphere):
5% Na2S·9H2O solution  8.0     ml