Medium data

Search for medium no. = [958].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

958   SEA SALTS TYG MEDIUM

Sea Salts (Sigma)  50.0     g
Wolfe's mineral elixir (see Medium No. 470)  2.0     ml
Trypticase peptone (BD-BBL)  1.0     g
Yeast extract  1.0     g
Glucose  1.0     g
Resazurin  0.5     mg
Na2CO3  1.0     g
Distilled water  1.0     L
Mix components thoroughly and autoclave under a N2-CO2 gas mixture. After cooling, adjust pH to 7.2 - 7.5 with 5% Na2CO3 solution (autoclaved and stored under N2). Then, add per litter the following solutions (autoclaved and stored under N2):
5% Na2S·9H2O solution  6.0     ml
5% L-Cysteine·HCl·H2O solution  6.0     ml