Medium data
Search for medium no. = [958].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
958 SEA SALTS TYG MEDIUM
| Sea Salts (Sigma) | 50.0 | g |
| Wolfe's mineral elixir (see Medium No. 470) | 2.0 | ml |
| Trypticase peptone (BD-BBL) | 1.0 | g |
| Yeast extract | 1.0 | g |
| Glucose | 1.0 | g |
| Resazurin | 0.5 | mg |
| Na2CO3 | 1.0 | g |
| Distilled water | 1.0 | L |
Mix components thoroughly and autoclave under a N2-CO2 gas mixture. After cooling, adjust pH to 7.2 - 7.5 with 5% Na2CO3 solution (autoclaved and stored under N2). Then, add per litter the following solutions (autoclaved and stored under N2):
| 5% Na2S·9H2O solution | 6.0 | ml |
| 5% L-Cysteine·HCl·H2O solution | 6.0 | ml |
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