Medium data
Search for medium no. = [953].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
953 IRD DESULFOCURVUS MEDIUM
KH2PO4 | 0.3 | g |
K2HPO4 | 0.3 | g |
NH4Cl | 1.0 | g |
NaCl | 2.0 | g |
KCl | 0.1 | g |
CaCl2·2H2O | 0.1 | g |
MgCl2·6H2O | 0.5 | g |
Trace element solution (see Medium No. 439) | 1.0 | ml |
Yeast extract | 1.0 | g |
Na2S2O3·5H2O | 3.16 | g |
L-Cysteine·HCl·H2O | 0.5 | g |
Resazurin | 0.5 | mg |
Distilled water | 1.0 | L |
Mix components thoroughly, adjust pH to 7.2 and autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, add the following solutions from anaerobic stocks (autoclaved or *filter-sterilized):
8% NaHCO3 solution* | 25.0 | ml |
1 M Sodium lactate solution | 20.0 | ml |
Aseptically distribute the medium into culture vessels under a stream of N2-CO2 (4:1, v/v), and seal with butyl rubber stoppers. Prior to inoculation, add per liter the following solution from an anaerobic stock (autoclave and store under a N2 atmosphere).
5% Na2S·9H2O solution | 8.0 | ml |
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