Medium data
Search for medium no. = [950].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
950 NH-JN4 MEDIUM
| Na2SO4 | 1.0 | g |
| NaCl | 30.0 | g |
| KCl | 0.7 | g |
| MgCl2·6H2O | 5.0 | g |
| CaCl2·2H2O | 1.0 | g |
| K2HPO4 | 0.4 | g |
| KH2PO4 | 0.4 | g |
| NH4Cl | 0.5 | g |
| (NH4)2Fe(SO4)2·6H2O | 2.0 | mg |
| Yeast extract (BD-Difco) | 3.0 | g |
| Tryptone (BD-Difco) | 4.0 | g |
| Sodium acetate | 6.8 | g |
| L-Cysteine·HCl·H2O | 0.6 | g |
| Trace element solution SL-10 (see Medium No. 433) | 1.0 | ml |
| Resazurin | 1.0 | mg |
| Distilled water | 960.0 | ml |
Mix components thoroughly and adjust pH to 6.8. Distribute the medium into culture vessels under a N2-CO2 (9:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add the following solution (per 960 ml) from anaerobic stock (filter-sterilized)
| 5% NaHCO3 solution | 40.0 | ml |
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