Medium data

Search for medium no. = [940].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.


KH2PO4  0.2     g
NH4Cl  0.25    g
NaCl  1.0     g
KCl  0.5     g
MgCl2·6H2O  0.4     g
CaCl2·2H2O  0.15    g
Sodium pyruvate  1.1     g
Yeast extract  2.0     g
FeCl2 solution (see Medium No. 187)  1.0     ml
Trace element solution (see Medium No. 187)  1.0     ml
Selenite tungstate solution (see Medium No. 431)  1.0     ml
Resazurin  0.4     mg
Distilled water  1.0     L
Mix components thoroughly and autoclave under a N2-CO2 (80:20, v/v) gas atmosphere. After cooling, add the following solutions from anaerobic stocks (filter-sterilized):
8% NaHCO3 solution  30.0     ml
Vitamin solution (see below)  1.0     ml
Distribute the medium into culture vessels under the same gas mixture. Finally, add per liter the following solution from an anaerobic stock:
5% Na2S·9H2O solution  7.0     ml
Vitamin solution:
p-Aminobenzoic acid  80.0     mg
Biotin  20.0     mg
Thiamine·HCl  200.0     mg
Vitamin B12  50.0     mg
Distilled water  1.0     L

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