Medium data
Search for medium no. = [940].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
940 AMINIVIBRIO ASINOENSIS MEDIUM
| KH2PO4 | 0.2 | g |
| NH4Cl | 0.25 | g |
| NaCl | 1.0 | g |
| KCl | 0.5 | g |
| MgCl2·6H2O | 0.4 | g |
| CaCl2·2H2O | 0.15 | g |
| Sodium pyruvate | 1.1 | g |
| Yeast extract | 2.0 | g |
| FeCl2 solution (see Medium No. 187) | 1.0 | ml |
| Trace element solution (see Medium No. 187) | 1.0 | ml |
| Selenite tungstate solution (see Medium No. 431) | 1.0 | ml |
| Resazurin | 0.4 | mg |
| Distilled water | 1.0 | L |
Mix components thoroughly and autoclave under a N2-CO2 (80:20, v/v) gas atmosphere. After cooling, add the following solutions from anaerobic stocks (filter-sterilized):
| 8% NaHCO3 solution | 30.0 | ml |
| Vitamin solution (see below) | 1.0 | ml |
Distribute the medium into culture vessels under the same gas mixture. Finally, add per liter the following solution from an anaerobic stock:
| 5% Na2S·9H2O solution | 7.0 | ml |
Vitamin solution:
| p-Aminobenzoic acid | 80.0 | mg |
| Biotin | 20.0 | mg |
| Thiamine·HCl | 200.0 | mg |
| Vitamin B12 | 50.0 | mg |
| Distilled water | 1.0 | L |
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