Medium data
Search for medium no. = [937].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
937 IRD ALKALIPHILUS MEDIUM
KH2PO4 | 0.3 | g |
K2HPO4 | 0.3 | g |
NH4Cl | 0.5 | g |
NaCl | 5.0 | g |
KCl | 0.1 | g |
CaCl2·2H2O | 0.1 | g |
MgCl2·6H2O | 0.5 | g |
Sodium acetate | 0.164 | g |
Trace minerals(see Medium No. 151) | 10.0 | ml |
Yeast extract | 5.0 | g |
Trypticase peptone (BD-BBL) | 5.0 | g |
L-Cysteine·HCl·H2O | 0.5 | g |
Resazurin | 1.0 | mg |
Distilled water | 885.0 | ml |
Mix components thoroughly, adjust pH to 8.5 and autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized):
8% NaHCO3 solution* | 75.0 | ml |
0.025 M Crotonic acid solution (pH 7.0) | 40.0 | ml |
Prior to inoculation, add per liter the following solution from an anaerobic stock (autoclaved and stored under a N2 atmosphere):
5% Na2S·9H2O solution | 8.0 | ml |
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