Medium data

Search for medium no. = [937].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

937   IRD ALKALIPHILUS MEDIUM

KH2PO4  0.3     g
K2HPO4  0.3     g
NH4Cl  0.5     g
NaCl  5.0     g
KCl  0.1     g
CaCl2·2H2O  0.1     g
MgCl2·6H2O  0.5     g
Sodium acetate  0.164   g
Trace minerals(see Medium No. 151)  10.0     ml
Yeast extract  5.0     g
Trypticase peptone (BD-BBL)  5.0     g
L-Cysteine·HCl·H2O  0.5     g
Resazurin  1.0     mg
Distilled water  885.0     ml
Mix components thoroughly, adjust pH to 8.5 and autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized):
8% NaHCO3 solution*  75.0     ml
0.025 M Crotonic acid solution (pH 7.0)  40.0     ml
Prior to inoculation, add per liter the following solution from an anaerobic stock (autoclaved and stored under a N2 atmosphere):
5% Na2S·9H2O solution  8.0     ml