Medium data
Search for medium no. = [929].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
929 HALANAEROBIUM SEHLINENSE MEDIUM
| KH2PO4 | 0.3 | g |
| K2HPO4 | 0.3 | g |
| NH4Cl | 1.0 | g |
| NaCl | 200.0 | g |
| KCl | 0.1 | g |
| CaCl2·2H2O | 0.1 | g |
| KNO3 | 2.0 | g |
| Trace minerals(see Medium No. 151) | 10.0 | ml |
| Yeast extract | 1.0 | g |
| Distilled water | 1.0 | L |
Add components to distilled water and bring volume to 1.0 L. Adjust pH to 8.3 with 10 M KOH. Bring to a boil and cool down under a N2 gas stream. Then, distribute the medium into culture vessels (e.g., 5 ml of the medium in the Hungate tubes) under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave at 110C for 20 min. Aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized) into the medium (per liter):
| 8% NaHCO3 solution* | 25.0 | ml |
| 10% MgCl2·6H2O solution | 15.0 | ml |
| 1 M Glucose solution | 20.0 | ml |
| 5% Na2S·9H2O solution | 8.0 | ml |
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