Medium data

Search for medium no. = [909].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

909   IRD MARINE CLOSTRIDA MEDIUM

KH2PO4  0.3     g
K2HPO4  0.3     g
NH4Cl  1.0     g
NaCl  23.0     g
KCl  0.1     g
CaCl2·2H2O  0.1     g
Trace minerals (see Medium No. 151)  10.0     ml
Yeast extract  2.0     g
L-Cysteine·HCl·H2O  0.5     g
Resazurin  1.0     mg
Distilled water  925.0     ml
Mix components thoroughly and autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, add the following solutions from anaerobic stocks (autoclaved or *filter-sterilized):
8% NaHCO3 solution*  25.0     ml
10% MgCl2·6H2O solution  30.0     ml
1 M Glucose solution  20.0     ml
Aseptically distribute the medium into culture vessels under a stream of N2-CO2 (4:1, v/v) and seal with butyl rubber stoppers. Prior to inoculation, add per liter the following solution from an anaerobic stock (autoclave and store under a N2 atmosphere):
5% Na2S·9H2O solution  8.0     ml