Medium data
Search for medium no. = [906].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
906 MK MEDIUM
| Trisodium citrate·2H2O | 1.0 | g |
| Tryptone (BD-Difco) | 2.0 | g |
| Peptone (Oxoid) | 1.0 | g |
| KH2PO4 | 0.6 | g |
| NH4Cl | 0.6 | g |
| NaCl | 200.0 | g |
| MgCl2·6H2O | 0.2 | g |
| K2SO4 | 20.0 | g |
| FeCl2·4H2O | 36.0 | mg |
| MnCl2·4H2O | 0.36 | mg |
| SL-6 trace element solution (see Medium No. 167) | 1.0 | ml |
Add components to distilled water and bring volume to 900 ml. Adjust pH to 7.0 and autoclave. For preparation of solid medium, add 20.0 g/L Bacto agar (BD-Difco). Separately autoclave the following solution:
| 0.5 M Glycine-NaOH buffer (pH 10.0) | 100.0 | ml |
After autoclaving, add the solution to the medium.
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