Medium data

Search for medium no. = [906].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

906   MK MEDIUM

Trisodium citrate·2H2O  1.0     g
Tryptone (BD-Difco)  2.0     g
Peptone (Oxoid)  1.0     g
KH2PO4  0.6     g
NH4Cl  0.6     g
NaCl  200.0     g
MgCl2·6H2O  0.2     g
K2SO4  20.0     g
FeCl2·4H2O  36.0     mg
MnCl2·4H2O  0.36    mg
SL-6 trace element solution (see Medium No. 167)  1.0     ml
Add components to distilled water and bring volume to 900 ml. Adjust pH to 7.0 and autoclave. For preparation of solid medium, add 20.0 g/L Bacto agar (BD-Difco). Separately autoclave the following solution:
0.5 M Glycine-NaOH buffer (pH 10.0)  100.0     ml
After autoclaving, add the solution to the medium.