Medium data
Search for medium no. = [892].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
892 MMJYP2 FOR AC55
| Yeast extract | 4.0 | g |
| Tryptone (BD-Difco) | 1.0 | g |
| Modified MJ synthetic seawater B (see below) | 1.0 | L |
Mix components and adjust pH to 7.0. Distribute the medium into culture vessels under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions from anaerobic stocks (*filter-sterilized or autoclaved):
| 8% NaHCO3 solution* | 12.5 | ml |
| 5% Na2S·9H2O solution (pH 7.5) | 10.0 | ml |
Pressurize the inoculated vessels to 200 kPa with the same gas mixture.
Modified MJ synthetic seawater B:
| NaCl | 25.0 | g |
| MgCl2·6H2O | 4.2 | g |
| MgSO4·7H2O | 3.4 | g |
| KCl | 0.5 | g |
| NH4Cl | 0.25 | g |
| K2HPO4 | 0.14 | g |
| CaCl2·2H2O | 0.7 | g |
| NiCl2·6H2O | 1.0 | mg |
| Na2SeO3·5H2O | 1.0 | mg |
| Trace mineral solution (see below) | 10.0 | ml |
| Distilled water | 1.0 | L |
Trace mineral solution:
| Trace minerals (see Medium No. 151) | 1.0 | L |
| NiCl2·6H2O | 0.025 | g |
| Na2SeO3·5H2O | 0.3 | mg |
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