Medium data

Search for medium no. = [892].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

892   MMJYP2 FOR AC55

Yeast extract  4.0     g
Tryptone (BD-Difco)  1.0     g
Modified MJ synthetic seawater B (see below)  1.0     L
Mix components and adjust pH to 7.0. Distribute the medium into culture vessels under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions from anaerobic stocks (*filter-sterilized or autoclaved):
8% NaHCO3 solution*  12.5     ml
5% Na2S·9H2O solution (pH 7.5)  10.0     ml
Pressurize the inoculated vessels to 200 kPa with the same gas mixture.
Modified MJ synthetic seawater B:
NaCl  25.0     g
MgCl2·6H2O  4.2     g
MgSO4·7H2O  3.4     g
KCl  0.5     g
NH4Cl  0.25    g
K2HPO4  0.14    g
CaCl2·2H2O  0.7     g
NiCl2·6H2O  1.0     mg
Na2SeO3·5H2O  1.0     mg
Trace mineral solution (see below)  10.0     ml
Distilled water  1.0     L
Trace mineral solution:
Trace minerals (see Medium No. 151)  1.0     L
NiCl2·6H2O  0.025   g
Na2SeO3·5H2O  0.3     mg

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