Medium data
Search for medium no. = [875].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
875 PHOSPHOAC3 MEDIUM
| KH2PO4 | 0.3 | g |
| K2HPO4 | 0.3 | g |
| NH4Cl | 1.0 | g |
| NaCl | 2.0 | g |
| KCl | 0.1 | g |
| CaCl2·2H2O | 0.1 | g |
| MgCl2·6H2O | 0.5 | g |
| Sodium acetate | 0.2 | g |
| Trace minerals (see Medium No. 151) | 10.0 | ml |
| Yeast extract | 2.0 | g |
| Sulfur (powder) | 10.0 | g |
| L-Cysteine·HCl·H2O | 0.5 | g |
| Resazurin | 1.0 | mg |
| Distilled water | 1.0 | L |
Mix components except sulfur, and autoclave under a N2-CO2 (4:1, v/v) gas mixture. Steam sulfur for 3 hr on each of 3 successive days. Aseptically distribute the medium into culture vessels containing appropriate amount of sulfur while gassing with a N2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Add per liter the following solutions (autoclaved and stored under N2, or *filter-sterilized and stored under N2-CO2, 4:1, v/v):
| 8% NaHCO3 solution* | 25.0 | ml |
| 1 M L-Sodium lactate solution | 20.0 | ml |
| 5% Na2S·9H2O solution | 8.0 | ml |
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