Medium data
Search for medium no. = [874].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
874 BELH1 MEDIUM
| KH2PO4 | 0.1 | g |
| K2HPO4 | 0.1 | g |
| NH4Cl | 1.0 | g |
| NaCl | 70.0 | g |
| KCl | 0.1 | g |
| CaCl2·2H2O | 0.1 | g |
| Trace element solution (see Medium No. 439) | 10.0 | ml |
| Yeast extract | 1.0 | g |
| Na2S2O3·5H2O | 5.0 | g |
| Resazurin | 1.0 | mg |
| Distilled water | 935.0 | ml |
Mix components thoroughly, adjust pH to 7.5 with KOH and autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, add the following solutions (autoclaved and stored under N2, or *filter-sterilized and stored under N2-CO2, 4:1, v/v):
| 1 M Glucose solution | 20.0 | ml |
| 8% NaHCO3 solution* | 25.0 | ml |
| 15% MgCl2·6H2O solution | 20.0 | ml |
Aseptically distribute the medium into culture vessels under the same gas mixture and seal with butyl rubber stoppers. Finally, add per liter the following solutions (autoclaved and stored under N2):
| 5% L-Cysteine·HCl·H2O solution | 10.0 | ml |
| 5% Na2S·9H2O solution | 8.0 | ml |
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