Medium data
Search for medium no. = [847].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
847 C5BEL MEDIUM
| KH2PO4 | 0.3 | g |
| K2HPO4 | 0.3 | g |
| NH4Cl | 0.5 | g |
| NaCl | 1.0 | g |
| KCl | 0.1 | g |
| CaCl2·2H2O | 0.1 | g |
| MgCl2·6H2O | 0.06 | g |
| Trace minerals (see Medium No. 151) | 10.0 | ml |
| Yeast extract | 1.0 | g |
| Resazurin | 1.0 | mg |
| Distilled water | 1.0 | L |
Mix components thoroughly and adjust pH to 7.5 with KOH. Distribute the medium into culture vessels (e.g., 5 ml of the medium in the Hungate tubes) under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add per liter the following solutions from anaerobic stocks (autoclaved or *filter-sterilized):
| 1.0 M Glucose solution | 20.0 | ml |
| 8% NaHCO3 solution* | 25.0 | ml |
| 5% Na2S·9H2O solution | 8.0 | ml |
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