Medium data
Search for medium no. = [844].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
844 RSULFAC1 MEDIUM
| KH2PO4 | 0.3 | g |
| K2HPO4 | 0.3 | g |
| NH4Cl | 1.0 | g |
| NaCl | 5.0 | g |
| KCl | 0.1 | g |
| CaCl2·2H2O | 0.1 | g |
| MgCl2·6H2O | 0.5 | g |
| Trace element solution (see Medium No. 439) | 10.0 | ml |
| Yeast extract | 1.0 | g |
| Peptone | 2.0 | g |
| L-Cysteine·HCl·H2O | 0.5 | g |
| Sulfur (powder) | 10.0 | g |
| Resazurin | 1.0 | mg |
| Distilled water | 1.0 | L |
Mix components except sulfur thoroughly and autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, add per liter the following solutions (autoclaved or *filter-sterilized):
| 8% NaHCO3 solution* | 25.0 | ml |
| 1.0 M Sodium fumarate solution | 20.0 | ml |
Steam sulfur for 3 hr on each of 3 successive days. Aseptically distribute the medium and sulfur into culture vessels under a stream of N2-CO2 (4:1, v/v) and seal with butyl rubber stoppers. Prior to inoculation, add per liter the following solution from an anaerobic stock (autoclaved and stored under a N2 atmosphere):
| 5% Na2S·9H2O solution | 8.0 | ml |
Copyright © 2024 Microbe Division (JCM) - All Rights Reserved