Medium data
Search for medium no. = [843].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
843 LIND BHT MEDIUM
| KH2PO4 | 0.3 | g |
| K2HPO4 | 0.3 | g |
| NH4Cl | 1.0 | g |
| NaCl | 20.0 | g |
| KCl | 0.1 | g |
| CaCl2·2H2O | 0.1 | g |
| Trace element solution (see Medium No. 439) | 1.0 | ml |
| Yeast extract | 2.0 | g |
| Na2S2O3·5H2O | 4.96 | g |
| L-Cysteine·HCl·H2O | 0.5 | g |
| Resazurin | 1.0 | mg |
| Distilled water | 925.0 | ml |
Mix components thoroughly and autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, add the following solutions from anaerobic stocks (autoclaved or *filter-sterilized):
| 8% NaHCO3 solution* | 25.0 | ml |
| 10% MgCl2·6H2O solution | 30.0 | ml |
| 1.0 M Sodium acetate solution | 20.0 | ml |
Then, aseptically distribute the medium into culture vessels under a stream of H2-CO2 (4:1, v/v) and seal with butyl rubber stoppers. Prior to inoculation, add per liter the following solution from an anaerobic stock (autoclave and store under a N2 atmosphere):
| 5% Na2S·9H2O solution | 8.0 | ml |
Pressurize inoculated culture vessels to 200 kPa H2-CO2 (4:1, v/v).
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