Medium data

Search for medium no. = [841].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

841   PROPIONISPORA MEDIUM

KH2PO4  0.2     g
NH4Cl  0.25    g
NaCl  1.0     g
MgCl2·6H2O  0.4     g
CaCl2·2H2O  0.15    g
FeCl2 solution (see Medium No. 187)  1.0     ml
Trace element solution (see Medium No. 187)  1.0     ml
Resazurin  0.5     mg
Yeast extract (BD-Difco)  1.0     g
Distilled water  900.0     ml
Mix components thoroughly and autoclave under a N2-CO2 (4:1, v/v) gas mixture. Aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized):
10% Fructose solution  50.0     ml
5% NaHCO3 solution*  50.0     ml
5% Na2S·9H2O  6.0     ml
Adjust pH to 7.0 - 7.2, if necessary.