Medium data
Search for medium no. = [841].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
841 PROPIONISPORA MEDIUM
| KH2PO4 | 0.2 | g |
| NH4Cl | 0.25 | g |
| NaCl | 1.0 | g |
| MgCl2·6H2O | 0.4 | g |
| CaCl2·2H2O | 0.15 | g |
| FeCl2 solution (see Medium No. 187) | 1.0 | ml |
| Trace element solution (see Medium No. 187) | 1.0 | ml |
| Resazurin | 0.5 | mg |
| Yeast extract (BD-Difco) | 1.0 | g |
| Distilled water | 900.0 | ml |
Mix components thoroughly and autoclave under a N2-CO2 (4:1, v/v) gas mixture. Aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized):
| 10% Fructose solution | 50.0 | ml |
| 5% NaHCO3 solution* | 50.0 | ml |
| 5% Na2S·9H2O | 6.0 | ml |
Adjust pH to 7.0 - 7.2, if necessary.
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