Medium data

Search for medium no. = [826].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

826   MODIFIED CM3 MEDIUM

(NH4)2S4  1.3     g
KH2PO4  1.5     g
K2HPO4  2.0     g
MgCl2·6H2O  0.5     g
CaCl2·2H2O  0.075   g
FeSO4·7H2O  1.25    mg
FeCl2 solution (see Medium No. 187)  1.0     ml
Trace element solution (see Medium No. 187)  1.0     ml
Yeast extract  2.0     g
L-Cysteine·HCl·H2O  0.5     g
Resazurin  1.0     g
Distilled water  950.0     ml
Mix components thoroughly and adjust pH to 6.0 with HCl. Autoclave under a N2 atmosphere. After cooling, aseptically and anaerobically add the following solution (filter-sterilized and stored under a N2 atmosphere):
10% Glucose solution  50.0     ml
Finally, adjust pH to 7.0 with sterile anoxic 5% Na2CO3 solution (usually 30 - 40 ml to 1.0 L medium).



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