Medium data
Search for medium no. = [824].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
824 DESULFOHALOBIUM MEDIUM
Solution A:
| NH4Cl | 1.0 | g |
| KH2PO4 | 0.3 | g |
| K2HPO4 | 0.3 | g |
| NaCl | 100.0 | g |
| KCl | 4.0 | g |
| Na2SO4 | 3.0 | g |
| Sodium acetate | 1.0 | g |
| Sodium lactate | 1.0 | g |
| Yeast extract | 1.0 | g |
| Trypticase peptone (BD-BBL) | 1.0 | g |
| FeCl2 solution (see Medium No. 187) | 1.0 | ml |
| Trace element solution (see Medium No. 187) | 1.0 | ml |
| Na2SeO3·5H2O | 3.0 | µg |
| Resazurin | 1.0 | mg |
| Distilled water | 900.0 | ml |
Mix components thoroughly and adjust pH to 7.0. Autoclave under a N2 atmosphere.
Solution B:
| MgCl2·6H2O | 20.0 | g |
| CaCl2·2H2O | 2.7 | g |
| Distilled water | 100.0 | ml |
Autoclave under a N2 atmosphere.
To complete the medium, combine Solutions A and B under a N2 atmosphere. Then, add per liter the following solution from an anaerobic stock:
| 3% Na2S·9H2O solution | 10.0 | ml |
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