Medium data
Search for medium no. = [796].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
796 DESULFITOCELLA MEDIUM
| KH2PO4 | 0.3 | g |
| K2HPO4 | 0.3 | g |
| NH4Cl | 1.0 | g |
| NaCl | 1.0 | g |
| MgCl2·6H2O | 0.5 | g |
| CaCl2·2H2O | 0.1 | g |
| KCl | 0.1 | g |
| Trace element solution (see Medium No. 483) | 1.0 | ml |
| Yeast extract | 1.0 | g |
| Trypticase peptone (BD-BBL) | 1.0 | g |
| L-Cysteine·HCl·H2O | 0.5 | g |
| Resazurin | 1.0 | mg |
| Distilled water | 925.0 | ml |
Mix components thoroughly, adjust pH to 7.0 with KOH, distribute the medium into culture vessels under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add per 925 ml the following solutions from anaerobic stocks (autoclaved or *filter-sterilized):
| 8% NaHCO3 solution* | 25.0 | ml |
| 0.1 M Sodium sulfite solution | 20.0 | ml |
| 1 M Sodium pyruvate solution | 20.0 | ml |
| Sodium dithionite solution (100 mg/L)* | 2.0 | ml |
| 5% Na2S·9H2O solution | 8.0 | ml |
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