Medium data
Search for medium no. = [483].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
483 ETHANOLOGENBACTERIUM MEDIUM
| Glucose | 10.0 | g |
| Tryptone (BD-Difco) | 4.0 | g |
| Beef extract (BD-Difco) | 2.0 | g |
| Yeast extract (BD-Difco) | 1.0 | g |
| NaCl | 4.0 | g |
| K2HPO4 | 1.5 | g |
| FeSO4·7H2O | 0.1 | g |
| MgCl2·6H2O | 0.2 | g |
| L-Cysteine·HCl·H2O | 0.5 | g |
| Microelement solution (see below) | 10.0 | ml |
| Vitamin solution (see below) | 10.0 | ml |
| Distilled water | 1.0 | L |
Mix components except vitamin solution, and adjust pH to 4.5 - 6.5. After autoclaving, aseptically add filter-sterilized vitamin solution.
Microelement solution:
| MnSO4·xH2O | 0.01 | g |
| ZnSO4·7H2O | 0.05 | g |
| H3BO3 | 0.01 | g |
| Nitrilotriacetic acid | 4.5 | g |
| CaCl2·2H2O | 0.01 | g |
| Na2MoO4·2H2O | 0.01 | g |
| CoCl2·6H2O | 0.2 | g |
| AlK(SO4)2·12H2O | 0.01 | g |
| Distilled water | 1.0 | L |
Vitamin solution:
| Cyanocobalamin | 0.01 | g |
| Ascorbic acid | 0.025 | g |
| Riboflavin | 0.025 | g |
| Citric acid | 0.02 | g |
| Pyridoxine·HCl | 0.05 | g |
| Folic acid | 0.01 | g |
| p-Aminobenzoic acid | 0.01 | g |
| Creatine | 0.025 | g |
| Distilled water | 1.0 | L |
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