Medium data

Search for medium no. = [483].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

483   ETHANOLOGENBACTERIUM MEDIUM

Glucose  10.0     g
Tryptone (BD-Difco)  4.0     g
Beef extract (BD-Difco)  2.0     g
Yeast extract (BD-Difco)  1.0     g
NaCl  4.0     g
K2HPO4  1.5     g
FeSO4·7H2O  0.1     g
MgCl2·6H2O  0.2     g
L-Cysteine·HCl·H2O  0.5     g
Microelement solution (see below)  10.0     ml
Vitamin solution (see below)  10.0     ml
Distilled water  1.0     L
Mix components except vitamin solution, and adjust pH to 4.5 - 6.5. After autoclaving, aseptically add filter-sterilized vitamin solution.
Microelement solution:
MnSO4·xH2O  0.01    g
ZnSO4·7H2O  0.05    g
H3BO3  0.01    g
Nitrilotriacetic acid  4.5     g
CaCl2·2H2O  0.01    g
Na2MoO4·2H2O  0.01    g
CoCl2·6H2O  0.2     g
AlK(SO4)2·12H2O  0.01    g
Distilled water  1.0     L
Vitamin solution:
Cyanocobalamin  0.01    g
Ascorbic acid  0.025   g
Riboflavin  0.025   g
Citric acid  0.02    g
Pyridoxine·HCl  0.05    g
Folic acid  0.01    g
p-Aminobenzoic acid  0.01    g
Creatine  0.025   g
Distilled water  1.0     L



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