Medium data

Search for medium no. = [791].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

791   S200 MEDIUM

NH4Cl  1.0     g
KH2PO4  0.5     g
CaCl2·2H2O  0.2     g
NaCl  150.0     g
MgCl2·6H2O  10.0     g
KCl  2.0     g
Yeast extract  0.5     g
Trace minerals (see Medium No. 151)  10.0     ml
L-Cysteine·HCl·H2O  0.5     g
Resazurin  1.0     mg
Add components to distilled water and bring volume to 1.0 L. Adjust pH to 7.0 with KOH, distribute the medium into culture vessels (e.g., 5 ml of the medium in the Hungate tubes) under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add per liter the following solutions from anaerobic stocks (autoclaved or *filter-sterilized):
8% NaHCO3 solution*  25.0     ml
1 M Glucose solution  20.0     ml
5% Na2S·9H2O solution  8.0     ml

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