Medium data
Search for medium no. = [774].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
774 MINERAL MEDIUM FOR HYDROGENOPHILUS ISLANDICUM
| KH2PO4 | 2.30 | g |
| Na2HPO4·6H2O | 2.9 | g |
| NH4Cl | 1.0 | g |
| NaHCO3 | 0.5 | g |
| CaCl2·2H2O | 0.1 | g |
| SL-6 trace element solution (see Medium No. 167) | 5.0 | ml |
| Distilled water | 880.0 | ml |
Mix components thoroughly, adjust pH to 6.8 and autoclave. After cooling, add the following solutions (autoclaved):
| 0.5% MgSO4·7H2O solution | 100.0 | ml |
| 0.1% Fe(NH4) citrate solution | 20.0 | ml |
Aseptically distribute the medium into culture vessels (e.g., 20 ml in 120 ml serum bottles) and seal with butyl rubber stoppers. Then, pressurize to 100 kPa with H2-CO2 (4:1, v/v).
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