Medium data

Search for medium no. = [765].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

765   DESULFOVIBRIO OCEANI MEDIUM

Na2SO4  3.0     g
KH2PO4  0.2     g
NH4Cl  0.3     g
NaCl  21.0     g
MgCl2·6H2O  3.0     g
KCl  0.5     g
CaCl2·2H2O  0.15    g
FeCl2 solution (see Medium No. 187)  1.0     ml
Trace element solution (see Medium No. 187)  1.0     ml
Selenite-tungstate solution (see Medium No. 431)  1.0     ml
Resazurin  1.0     mg
Distilled water  950.0     ml
Mix components thoroughly and autoclave under a N2-CO2 (4:1, v/v) atmosphere. After cooling, add the following sterile solutions (autoclaved or *filter-sterilized):
10% L-Sodium lactate solution  12.5     ml
Vitamin solution (see Medium No. 403)*  2.0     ml
Thiamine solution (see Medium No. 403)*  2.0     ml
Vitamin B12 solution (see Medium No. 403)*  2.0     ml
8% NaHCO3 solution*  31.5     ml
Finally, reduce the medium with the following solution (autoclaved and stored under a N2 atmosphere):
5% Na2S·9H2Osolution  8.0     ml



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