Medium data
Search for medium no. = [765].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
765 DESULFOVIBRIO OCEANI MEDIUM
| Na2SO4 | 3.0 | g |
| KH2PO4 | 0.2 | g |
| NH4Cl | 0.3 | g |
| NaCl | 21.0 | g |
| MgCl2·6H2O | 3.0 | g |
| KCl | 0.5 | g |
| CaCl2·2H2O | 0.15 | g |
| FeCl2 solution (see Medium No. 187) | 1.0 | ml |
| Trace element solution (see Medium No. 187) | 1.0 | ml |
| Selenite-tungstate solution (see Medium No. 431) | 1.0 | ml |
| Resazurin | 1.0 | mg |
| Distilled water | 950.0 | ml |
Mix components thoroughly and autoclave under a N2-CO2 (4:1, v/v) atmosphere. After cooling, add the following sterile solutions (autoclaved or *filter-sterilized):
| 10% L-Sodium lactate solution | 12.5 | ml |
| Vitamin solution (see Medium No. 403)* | 2.0 | ml |
| Thiamine solution (see Medium No. 403)* | 2.0 | ml |
| Vitamin B12 solution (see Medium No. 403)* | 2.0 | ml |
| 8% NaHCO3 solution* | 31.5 | ml |
Finally, reduce the medium with the following solution (autoclaved and stored under a N2 atmosphere):
| 5% Na2S·9H2Osolution | 8.0 | ml |
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