Medium data
Search for medium no. = [759].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
759 DETHIOSULFOVIBRIO PEPTIDOVORANS MEDIUM
| NH4Cl | 1.0 | g |
| KH2PO4 | 0.3 | g |
| K2HPO4 | 0.3 | g |
| MgCl2·6H2O | 3.0 | g |
| CaCl2·2H2O | 0.1 | g |
| KCl | 0.1 | g |
| NaCl | 30.0 | g |
| Sodium acetate | 0.5 | g |
| Yeast extract | 1.0 | g |
| Trypticase peptone (BD-BBL) | 5.0 | g |
| Trace minerals (see Medium No. 151) | 10.0 | ml |
| Resazurin | 0.5 | mg |
| Distilled water | 900.0 | ml |
Mix components and bubble with N2-CO2 (4:1, v/v) gas mixture. Distribute the medium into tubes or bottles under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add the following solutions from sterile anaerobic stocks (autoclaved or *filter-sterilized; per 900 ml of medium):
| 8% NaHCO3 solution* | 60.0 | ml |
| 12.5% Na2S2O3·5H2O solution* | 40.0 | ml |
| 3% Na2S·9H2O solution | 15.0 | ml |
Readjust pH of the medium to 7.3, if necessary.
Copyright © 2024 Microbe Division (JCM) - All Rights Reserved