Medium data
Search for medium no. = [757].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
757 DESULFOVIBRIO MEXICANUS MEDIUM
| NH4Cl | 1.0 | g |
| KH2PO4 | 0.3 | g |
| K2HPO4 | 0.3 | g |
| MgCl2·6H2O | 0.2 | g |
| CaCl2·2H2O | 0.1 | g |
| KCl | 0.1 | g |
| NaCl | 0.6 | g |
| Yeast extract | 1.0 | g |
| FeCl2 solution (see Medium No. 187) | 1.0 | ml |
| Trace element solution (see Medium No. 187) | 1.0 | ml |
| L-Cysteine·HCl·H2O | 0.5 | g |
| Resazurin | 0.5 | mg |
| Distilled water | 900.0 | ml |
Mix components and adjust pH to 7.0. Distribute the medium into tubes or bottles under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers, and autoclave. After cooling, add per liter (final) the following solutions from sterile anaerobic stocks (autoclaved or *filter-sterilized):
| 8% NaHCO3 solution* | 50.0 | ml |
| Substrate solution (see below) | 100.0 | ml |
| 3% Na2S·9H2O solution | 10.0 | ml |
Readjust pH of the medium to 7.0, if necessary.
Substrate solution:
| L-Sodium lactate | 12.5 | g |
| Na2S2O3·5H2O | 12.5 | g |
| Distilled water | 100.0 | ml |
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