Medium data
Search for medium no. = [741].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
741 ACETOBACTERIUM MEDIUM
| NH4Cl | 1.0 | g |
| KH2PO4 | 0.33 | g |
| K2HPO4 | 0.45 | g |
| MgSO4·7H2O | 0.1 | g |
| Trace element solution (see below) | 20.0 | ml |
| Trace vitamins (see Medium No. 197) | 20.0 | ml |
| Yeast extract | 2.0 | g |
| Fructose | 10.0 | g |
| Resazurin | 1.0 | mg |
| NaHCO3 | 10.0 | g |
| L-Cysteine·HCl·H2O | 0.5 | g |
| Na2S·9H2O | 0.5 | g |
| Distilled water | 1.0 | L |
Dissolve ingredients except NaHCO3, fructose, cysteine and sodium sulfide, bring to a boil for a few minutes and cool to room temperature under a N2-CO2 (80:20, v/v) gas mixture. Add NaHCO3 (solid) and equilibrate the medium with the gas until pH reaches ca. 7.4. Then distribute and autoclave under the same gas atmosphere. Before use, adjust pH to 8.2 by adding sterile anaerobic Na2CO3 solution (ca. 0.25 ml of 5% Na2CO3 per 10 ml medium) and add fructose, cysteine and sodium sulfide from anaerobic sterile stock solutions.
Trace element solution:
| Nitrilotriacetic acid | 1.5 | g |
| MgSO4·7H2O | 3.0 | g |
| MnSO4·xH2O | 0.5 | g |
| NaCl | 1.0 | g |
| FeSO4·7H2O | 0.1 | g |
| CoSO4·7H2 | 0.18 | g |
| CaCl2·2H2O | 0.1 | g |
| ZnSO4·7H2O | 0.18 | g |
| CuSO4·5H2O | 10.0 | mg |
| KAl(SO4)2·12H2O | 20.0 | mg |
| H3BO3 | 10.0 | mg |
| Na2MoO4·2H2O | 10.0 | mg |
| NiCl2·6H2O | 25.0 | mg |
| Na2SeO3·5H2O | 0.3 | mg |
| Distilled water | 1.0 | L |
Dissolve nitrilotriacetic acid at first and adjust pH to 6.5 with KOH, then add the remaining minerals.
Adjust final pH of the medium to 7.0 with KOH.
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