Medium data
Search for medium no. = [721].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
721 THIOALBUS DENITRIFICANS MEDIUM
| NaCl | 20.0 | g |
| MgCl2·6H2O | 0.4 | g |
| CaCl2·2H2O | 0.1 | g |
| NH4Cl | 0.2 | g |
| KH2PO4 | 1.0 | g |
| KCl | 0.5 | g |
| Yeast extract | 0.1 | g |
| Distilled water | 1.0 | L |
Mix components and adjust pH to 7.5. Dispense the medium into culture vessels, purge with N2 for 5 min, seal with butyl rubber stoppers and autoclave. After cooling, add per liter the following solutions (filter-sterilized and stored anaerobically):
| Vitamin solution (see Medium No. 403) | 1.0 | ml |
| Trace element solution (see Medium No. 439) | 1.0 | ml |
| 8% NaHCO3 solution | 5.0 | ml |
| 1 M NaNO3 solution | 5.0 | ml |
| 1 M Na2S2O3 solution | 5.0 | ml |
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