Medium data
Search for medium no. = [716].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
716 USAB 53 MEDIUM
| NH4Cl | 1.0 | g |
| KH2PO4 | 0.3 | g |
| K2HPO4 | 0.3 | g |
| NaCl | 25.0 | g |
| CaCl2·2H2O | 0.1 | g |
| KCl | 0.1 | g |
| Na2SO4 | 2.84 | g |
| Trace mineral solution (see Medium No. 684) | 1.0 | ml |
| Yeast extract | 1.0 | g |
| Sodium lactate | 2.24 | g |
| Resazurin | 1.0 | mg |
| Distilled water | 1.0 | L |
Mix components and adjust pH to 6.8 - 7.0 with NaOH. Bring to a boil, cool down under a N2 gas stream and add 2 ml of 5% Na2S·9H2O solution. Anaerobically distribute the medium into culture vessels, seal with butyl rubber stoppers and autoclave.
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