Medium data

Search for medium no. = [708].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

708 DEFINED MEDIUM FOR SULFATE-REDUCING BACTERIA WITH BUTYRATE

KH₂PO₄	`0.5`	g
NH₄Cl	`1.0`	g
CaCl₂·2H₂O	`0.1`	g
MgSO₄·7H₂O	`2.5`	g
NaCl	`1.0`	g
FeCl₂ solution (see Medium No. 187)	`10.0`	ml
Trace element solution (see Medium No. 187)	`10.0`	ml
Resazurin	`1.0`	mg
Distilled water	`870.0`	ml

Mix ingredients and adjust pH to 7.5. Distribute the medium into culture vessels under a N₂-CO₂ (95:5, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. Anaerobically and aseptically add per liter the following solutions (autoclaved or *filter-sterilized) to the medium:

2.2% Sodium butyrate solution	`100.0`	ml
8% NaHCO₃ solution*	`2.5`	ml
5% L-Cysteine·H₂O·HCl solution	`10.0`	ml

Readjust pH of the medium to 7.5, if necessary.
For preparation of solid medium, add 15.0 g/L agar (BD-Difco).