Medium data

Search for medium no. = [707].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

707 LACTATE SULFATE MEDIUM

Yeast extract	`1.0`	g
Na₂SO₄	`7.1`	g
L-Sodium lactate	`6.95`	g
MgSO₄·7H₂O	`1.97`	g
NH₄Cl	`1.08`	g
K₂HPO₄	`0.38`	g
CaCl₂·2H₂O	`0.09`	g
Trace vitamins (see Medium No. 197)	`2.0`	ml
0.01% Coenzyme M solution	`0.2`	ml
Vitamin B₁₂ solution (see Medium No. 403)	`0.1`	ml
Trace element solution (see Medium No. 301)	`12.5`	ml
HEPES	`5.96`	g
Resazurin	`1.0`	mg
Distilled water	`1.0`	L

Adjust pH to 7.2.
Autoclave under a N₂ gas atmosphere. After cooling, add the following sterile solutions (autoclaved or *filter-sterilized):

7% NaHCO₃ solution*	`10.0`	ml
2.5% L-Cysteine·HCl·H₂O solution	`10.0`	ml