Medium data

Search for medium no. = [706].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

706 SPOROSALIBACTERIUM MEDIUM

KH₂PO₄	`0.3`	g
K₂HPO₄	`0.3`	g
NH₄Cl	`1.0`	g
NaCl	`40.0`	g
CaCl₂·2H₂O	`0.2`	g
Yeast extract	`2.0`	g
Trypticase peptone (BD-BBL)	`2.0`	g
Glucose	`3.6`	g
Trace element solution (see Medium No. 439)	`1.0`	ml
Resazurin	`1.0`	mg
Distilled water	`940.0`	ml

Mix ingredients and adjust pH to 7.2. Distribute the medium into culture vessels (e.g., 4.7 ml of the medium in the Hungate tubes) under a N₂-CO₂ (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave.
To complete the medium, add the following stock solutions [Filter-sterilize NaHCO₃ solution and store under a N₂-CO₂ (4:1, v/v) gas mixture. The other solutions are autoclaved and stored anaerobically under a N₂ atmosphere] to the medium (per 4.7 ml of the medium):

15% MgCl₂·6H₂O solution	`0.1`	ml
5% NaHCO₃ solution	`0.2`	ml
5% L-Cysteine·HCl·H₂O	`0.05`	ml
5% Na₂S·9H₂O solution	`0.04`	ml