Medium data
Search for medium no. = [706].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
706 SPOROSALIBACTERIUM MEDIUM
KH2PO4 | 0.3 | g |
K2HPO4 | 0.3 | g |
NH4Cl | 1.0 | g |
NaCl | 40.0 | g |
CaCl2·2H2O | 0.2 | g |
Yeast extract | 2.0 | g |
Trypticase peptone (BD-BBL) | 2.0 | g |
Glucose | 3.6 | g |
Trace element solution (see Medium No. 439) | 1.0 | ml |
Resazurin | 1.0 | mg |
Distilled water | 940.0 | ml |
Mix ingredients and adjust pH to 7.2. Distribute the medium into culture vessels (e.g., 4.7 ml of the medium in the Hungate tubes) under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave.
To complete the medium, add the following stock solutions [Filter-sterilize NaHCO3 solution and store under a N2-CO2 (4:1, v/v) gas mixture. The other solutions are autoclaved and stored anaerobically under a N2 atmosphere] to the medium (per 4.7 ml of the medium):
15% MgCl2·6H2O solution | 0.1 | ml |
5% NaHCO3 solution | 0.2 | ml |
5% L-Cysteine·HCl·H2O | 0.05 | ml |
5% Na2S·9H2O solution | 0.04 | ml |
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