Medium data

Search for medium no. = [706].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

706   SPOROSALIBACTERIUM MEDIUM

KH2PO4  0.3     g
K2HPO4  0.3     g
NH4Cl  1.0     g
NaCl  40.0     g
CaCl2·2H2O  0.2     g
Yeast extract  2.0     g
Trypticase peptone (BD-BBL)  2.0     g
Glucose  3.6     g
Trace element solution (see Medium No. 439)  1.0     ml
Resazurin  1.0     mg
Distilled water  940.0     ml
Mix ingredients and adjust pH to 7.2. Distribute the medium into culture vessels (e.g., 4.7 ml of the medium in the Hungate tubes) under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave.
To complete the medium, add the following stock solutions [Filter-sterilize NaHCO3 solution and store under a N2-CO2 (4:1, v/v) gas mixture. The other solutions are autoclaved and stored anaerobically under a N2 atmosphere] to the medium (per 4.7 ml of the medium):
15% MgCl2·6H2O solution  0.1     ml
5% NaHCO3 solution  0.2     ml
5% L-Cysteine·HCl·H2O  0.05    ml
5% Na2S·9H2O solution  0.04    ml