Medium data

Search for medium no. = [681].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

681   PFENNIG'S MEDIUM

K2HPO4  0.25    g
NH4Cl  0.34    g
KCl  0.34    g
MgSO4·7H2O  0.5     g
CaCl2·2H2O  0.25    g
Distilled water  1.0     L
Add the following components after autoclaving:
Trace element solution SL-10 (see Medium No. 433)  0.5     ml
Na2S·9H2O  0.3     g
NaHCO3  1.5     g
Vitamin B12  0.02    mg
Acetate (final concentration)  2.0     mM
Thiosulfate (final concentration)  10.0     mM
Adjust pH of the medium to 7.2.