Medium data
Search for medium no. = [678].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
678 HALANAEROBACULUM TUNISIENSE MEDIUM
KH2PO4 | 0.3 | g |
K2HPO4 | 0.3 | g |
NH4Cl | 1.0 | g |
NaCl | 250.0 | g |
KCl | 0.1 | g |
CaCl2·2H2O | 0.1 | g |
KNO3 | 2.0 | g |
Trace minerals (see Medium No. 151) | 10.0 | ml |
Yeast extract | 1.0 | g |
Distilled water | 1.0 | L |
Mix components and adjust pH to 8.3 with 10 M KOH. Bring to a boil and cool down under a N2 gas stream. Then, distribute the medium into culture vessels (e.g., 5 ml of the medium in the Hungate tubes) under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave at 110C for 45 min. Aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized) into the medium (per liter):
8% NaHCO3solution* | 25.0 | ml |
10% MgCl2·6H2O solution | 20.0 | ml |
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