Medium data

Search for medium no. = [650].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

650   THIOPHAEOCOCCUS MEDIUM

KH2PO4  0.5     g
CaCl2·2H2O  0.15    g
MgSO47H2O  2.0     g
NH4Cl  0.64    g
NaCl  20.0     g
Yeast extract  0.4     g
Sodium pyruvate  3.0     g
Trace element solution SL8 (see Medium No. 190)  1.0     ml
NaHCO3 (10%, w/v)  10.0     ml
Vitamin B12 (2 mg/ml)  1.0     ml
Na2S·9H2O (final conc.)  1.0     mM
Na2S2O3·5H2O (final conc.)  6.0     mM
Distilled water  1.0     L
Adjust pH to 7.5.
After autoclaving, add filter-sterilized vitamin B12, NaHCO3, Na2S·9H2O and Na2S2O3·5H2O solutions to the medium.