Medium data
Search for medium no. = [190].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
190 LYS MEDIUM
| KH2PO4 | 1.0 | g |
| (NH4)2SO4 | 1.0 | g |
| NaCl | 0.2 | g |
| MgCl2·6H2O | 0.2 | g |
| CaCl2·2H2O | 0.05 | g |
| Trace element solution SL8 (see below) | 1.0 | ml |
| Sodium lactate | 2.2 | g |
| Yeast extract | 0.5 | g |
| Vitamin B12 | 5.0 | µg |
| Distilled water | 1.0 | L |
Adjust pH to 6.8.
Trace element solution SL8:
| EDTA·2Na | 5.2 | g |
| FeCl2·4H2O | 1.5 | g |
| ZnCl2 | 70.0 | mg |
| MnCl2·4H2O | 100.0 | mg |
| H3BO3 | 62.0 | mg |
| CoCl2·6H2O | 190.0 | mg |
| CuCl2·2H2O | 17.0 | mg |
| NiCl2·6H2O | 24.0 | mg |
| Na2MoO4·2H2O | 36.0 | mg |
| Distilled water | 1.0 | L |
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