Medium data

Search for medium no. = [190].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

190   LYS MEDIUM

KH2PO4  1.0     g
(NH4)2SO4  1.0     g
NaCl  0.2     g
MgCl2·6H2O  0.2     g
CaCl2·2H2O  0.05    g
Trace element solution SL8 (see below)  1.0     ml
Sodium lactate  2.2     g
Yeast extract  0.5     g
Vitamin B12  5.0     µg
Distilled water  1.0     L
Adjust pH to 6.8.
Trace element solution SL8:
EDTA·2Na  5.2     g
FeCl2·4H2O  1.5     g
ZnCl2  70.0     mg
MnCl2·4H2O  100.0     mg
H3BO3  62.0     mg
CoCl2·6H2O  190.0     mg
CuCl2·2H2O  17.0     mg
NiCl2·6H2O  24.0     mg
Na2MoO4·2H2O  36.0     mg
Distilled water  1.0     L