Medium data

Search for medium no. = [622].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.


Solution A:
NaCl  30.0     g
K2HPO4  0.09    g
KH2PO4  0.07    g
KCl  0.33    g
NH4Cl  0.535   g
NaHCO3  2.0     g
Trace element solution (see below)  10.0     ml
Trace vitamins (see Medium No. 197)  10.0     ml
Resazurin  1.0     mg
Distilled water  900.0     ml
Mix components and adjust pH to 6.8. Distribute the medium into culture vessels (e.g., 18 ml in 50 ml serum bottle) under a stream of a N2-CO2 (80:20, v/v) gas mixture, seal with butyl rubber stoppers and autoclave.
Solution B:
CaCl2·2H2O  1.06    g
MgCl2·6H2O  4.18    g
Distilled water  100.0     ml
Mix components and autoclave under a N2 atmosphere.
Solution C:
10% yeast extract solution  10.0     ml
Autoclave under a N2 atmosphere.
Aseptically and anaerobically combine Solutions A, B and C in the culture vessels. Then, add (per liter) the following solutions (autoclaved and stored under a N2 atmosphere):
3% Na2S·9H2O solution  10.0     ml
3% L-Cysteine·HCl·H2O solution  10.0     ml
Comment: For strain JCM 14728, incubate with a 5% inoculum.

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