Medium data

Search for medium no. = [573].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

573   CLOSTRIDIISALIBACTER PAUCIVORANS MEDIUM

KH2PO4  0.3     g
K2HPO4  0.3     g
NH4Cl  1.0     g
NaCl  40.0     g
KCl  0.1     g
CaCl2  0.1     g
Yeast extract  2.0     g
Trace minerals (see Medium No. 151)  10.0     ml
Resazurin  1.0     mg
Distilled water  900.0     ml
Mix ingredients and adjust pH to 7.0. Distribute 0.9 volume of the medium into tubes (e.g., 4.5 ml of the medium in the Hungate tubes) under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave.
To complete the medium, add the following stock solutions [Filter-sterilize NaHCO3 solution and store under a N2-CO2 (4:1, v/v) gas mixture. The other solutions are autoclaved and stored anaerobically under a N2 atmosphere] to the medium (per 4.5 ml):
15% MgCl2·6H2O solution  0.1     ml
5% NaHCO3 solution  0.3     ml
2% Na2S·9H2O solution  0.1     ml