Medium data
Search for medium no. = [552].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
552 SULFATE REDUCING BACTERIUM MEDIUM WITH LACTATE
| KH2PO4 | 0.5 | g |
| NH4Cl | 1.0 | g |
| Na2SO4 | 1.0 | g |
| CaCl2·2H2O | 0.1 | g |
| MgSO4·7H2O | 2.0 | g |
| NaCl | 10.0 | g |
| Yeast extract (BD-Difco) | 0.5 | g |
| Vitamin solution (see below) | 1.0 | ml |
| FeCl2 solution (see Medium No. 187) | 1.0 | ml |
| Trace element solution (see Medium No. 187) | 1.0 | ml |
| L-Sodium lactate | 2.3 | g |
| Resazurin | 1.0 | mg |
| L-Cysteine·H2O·HCl | 0.5 | g |
| Distilled water | 1.0 | L |
Mix ingredients except L-sodium lactate and L-cysteine·H2O·HCl, adjust pH to 7.5 and autoclave under a N2 atmosphere. Separately autoclave L-sodium lactate and L-cysteine·H2O·HCl in 10 ml distilled water under a N2 atmosphere and add to the medium. Readjust pH to 7.5 with 1 N NaOH (from a sterile stock solution).
For preparation of solid medium, add 15 g/L agar (BD-Difco).
Vitamin solution:
| Biotin | 10.0 | µg |
| p-Aminobenzoic acid | 40.0 | µg |
| Nicotinic acid | 100.0 | µg |
| Distilled water | 1.0 | L |
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